1.       Busiest organ
Heart
2.       Fastest known enzyme
Carbonic anhydrase
3.       Hardest part of the body
Enamel of tooth
4.       Heaviest organ
Liver (1.6 kilograms)
5.       Heaviest part of the body
Skull
6.       Largest (bulkiest) muscle of human body
Gluteus maximus (Buttock muscle)
7.       Largest blood cell
Monocyte
8.       Largest blood vessel (Artery)
Aorta (abdominal aorta)
9.       Largest bone of man
Femur bone
10.   Largest cell
Egg of Ostrich (15- 20 cm in diameter)
11.   Largest cell in human body
Ovum (egg)
12.   Largest cell organelle
Nucleus
13.   Largest endocrine gland
Thyroid gland
14.   Largest enzyme
Catalase
15.   Largest excretory organ
Kidney
16.   Largest gland
Liver (1.5 kg)
17.   Largest nerve
10th cranial nerve (Vagus)
18.   Largest organ
Skin (4 kg; 24 cubic feet)
19.   Largest salivary gland
Parotid gland
20.   Largest sense organ
Skin
21.   Largest vein
Inferior vena cava
22.   Largest WBC
Monocyte
23.   Longest cell
Neuron (Nerve cell)
24.   Longest muscle
Sartorius (Thigh muscle)
25.   Longest nerve
Sciatic
26.   Longest part of the alimentary canal
Small intestine (7m)
27.   Most abundant compound in a cell
Water (Next to water protein)
28.   Most abundant mineral in vertebrate body
Ca (2%); followed by P (1%)
29.   Most flexible organ
Skin
30.   Part having least amount of water
Enamel of tooth
31.   Part having much amount of water
Blood
32.   Part having thickest skin
Sole of the foot (about 4.7 mm)
33.   Part having thinnest skin
Face (0.12 mm)
34.   Second largest gland
Pancreas
35.   Smallest artery
Phrenics (To diaphragm)
36.   Smallest blood vessels
Blood capillaries
37.   Smallest bone
Stapes of ear
38.   Smallest cell
Mycoplasma  gallisepticum (PPLO)
39.   Smallest cell of the vertebrate body
Squamous epithelial cells
40.   Smallest cell organelle
Ribosome (Found in maximum number)
41.   Smallest endocrine gland
Pituitary
42.   Smallest enzyme
Peroxidase
43.   Smallest face bone
Lachrymal bone
44.   Smallest muscle
Stapedius (near stapes)
45.   Smallest nerve
Pathetic trochlear nerve (4th cranial nerve)
46.   Smallest salivary gland
Sublingual gland
47.   Smallest WBC
Lymphocyte
48.   Strongest bone
Femur bone (not jaw bone)
49.   Sweetest protein
Monellin
50.   Sweetest sugar
Fructose
51.   Thinnest skin
Conjunctiva
52.   Hardest working muscle
Heart muscles
53.   Strongest muscle based on its weight
Masseter (Jaw muscles), somebody says it is Uterus
54.   Muscle that can pull with the greatest force
Soleus

Physiology and anatomy
Superlatives